Category: Conversion

Conversion webpage

Isobutanol Production in Engineered Saccharomyces cerevisiae by Overexpression of 2-ketoisovalerate decarboxylase and Valine Biosynthetic Enzymes.

In this study, construction of isobutanol production systems was attempted by overexpression of effective 2-keto acid decarboxylase (KDC) and combinatorial overexpression of valine biosynthetic enzymes in Saccharomyces cerevisiae D452-2.
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Enzymatic hydrolysis of cellulosic biomass

Enzymatic hydrolysis that converts lignocellulosic biomass to fermentable sugars may be the most complex step in this process due to substrate-related and enzyme-related effects and their interactions. This review looks at which organisms produce enzymes, as well as the chemistry and physics of these enzymatic processes.
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Research targets cells to reduce lignin, boost fermentable sugar

Researchers at the Joint BioEnergy Institute are exploring new ways to meet the challenge of recalcitrant cellulosic feedstocks and lower the cost of biomass-based biofuels. The group recently published two papers detailing their work to genetically modify xylan and lignin, resulting in improved conversion characteristics in the model plants used in the research.
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High-flux Isobutanol Production using Engineered Escherichia coli: A Bioreactor Study with in situ Product Removal

Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. This research translates the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains.
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