Category: Conversion

Conversion webpage

High-flux Isobutanol Production using Engineered Escherichia coli: A Bioreactor Study with in situ Product Removal

Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. This research translates the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains.
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Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose

Producing biofuels directly from cellulose, known as consolidated bioprocessing, is believed to reduce costs substantially compared to a process in which cellulose degradation and fermentation to fuel are accomplished in separate steps. This research presents a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose.
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Second-Generation Biofuel: Isobutanol Producing Biocatalyst

With support from EPA’s Small Business Innovation Research (SBIR) Program, Gevo launched a project to identify rate-limiting steps in the isobutanol pathway in yeast. This effort resulted in the development of genetic and biochemical assays to characterize each of the enzymes involved in the production of isobutanol in yeast.
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